Translational Research & Pharmacology Core Facility

Preclinical Drug Development & Testing Laboratory

Director: Afshin Dowlati, MD
Professor, Medicine-Hematology/Oncology
axd44@case.edu

Co-Director: John J. Pink, PhD
Assistant Professor, General Medical Sciences (Oncology)
john.pink@case.edu

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Summary of Services

The Preclinical Drug Development & Testing Laboratory provides the infrastructure to perform preclinical in vitro testing of novel agents or combinations of agents in cancer models. The overall goal of the laboratory is to provide high quality, validated preclinical data to members of the scientific community at Case Western Reserve who have a potential anticancer agent that they wish to further develop. The ultimate endpoint is to test/validate the efficacy of new agents and combinations that may result in the design/initiation of animal studies and eventually clinical trials.

The following services are available to be performed in cells lines from a variety of tumor types, including:

  • Breast
  • Melanoma
  • Colon
  • Ovarian
  • Cervical
  • Lung
    • Small Cell
    • Non-Small Cell

Services

Standard assays include, but are not limited to:

  • Growth/Viability Assays
    1. Single agent or combination studies:
      • Survival Assay (Colony Formation Assay)
      • Cell Viability Assay (MTS Assay)
      • Growth Inhibition Assay (DNA Assay)
    2. Radio-Chemosensitization (Combinations with gamma-irradiation)
      • Survival Assay (Colony Formation Assay)
      • Cell Viability Assay (MTS Assay)
      • Growth Inhibition Assay (DNA Assay)
  • Cell Response Assays
    1. Cell Cycle Progression/Arrest
      • Flow cytometry-PI
    2. Apoptosis
      • TUNEL assay
      • Caspase cleavage
      • PARP Cleavage
  • Mechanistic Studies
    1. Custom studies designed in collaboration with investigators

Fees

Please contact John Pink, PhD or Afshin Dowlati, MD for pricing information.


Contact for Information

Location: Wolstein Research Building 3-503
2103 Cornell Rd.
Cleveland, OH 44106-7285

Director: Afshin Dowlati, MD
216.844.1225
afshin.dowlati@case.edu

Co-Director: John Pink, PhD
216.368.5420
john.pink@case.edu

Lab Manager: Ian Lent
216.368.4178
ijl4@case.edu