Translational Research & Pharmacology Core Facility

Director: John J. Pink, PhD
Assistant Professor, General Medical Sciences (Oncology)
john.pink@case.edu

Co-Director (Pharmacology): Yan Xu, PhD
Professor, Chemistry
yan.xu@case.edu

CCF Site Director: Daniel J. Lindner, MD, PhD
Assistant Professor, Cancer Biology
lindned@ccf.org

Operations Director: Paul Hartman, MS
Manager of Dahms Clinical Research Unit Core Lab
phh@case.edu

  Translational Group Hohler

Services and Technologies

List of Services

  • Consultation on design and implementation of Correlative Studies for Clinical Trials
  • Sample Handling (receiving, tracking, storage, distribution)
  • Sample Processing (blood, tissue and paraffin block processing)
    • Routine Blood Processing (PBMC, serum, or plasma isolation)
    • DNA, RNA, protein extraction
    • Flow or laser scanning cytometry staining
  • Lab Analysis
    • Standard ELISAs (Fluorescent or Luminescent platforms)
    • MSD Multiplex Analysis (Mesoscale Electrochemiluminescence platform)
    • Comet assays
  • Preclinical Drug Testing
    • Single or multiple agent cytotoxicity (MTT or DNA content assays)
    • Multiple agent analysis (Synergy analysis)
    • Other single or multiple agent assays (e.g., flow cytometry)
    • Cell Line verification (STR analysis)
    • Mycoplasma testing
  • Pharmacokinetic assay development
    • Pharmacokinetic expertise for investigator-initiated early phase studies with planned PK analyses
    • Sample procurement schedules for pharmacokinetic assays given anticipated or known PK profiles of investigational agents
    • Design and development of analytical methods
    • Adoption and validation of established analytical procedures
  • Pharmacokinetic assay performance
    • Quantitative measurement of drugs, metabolites, and other small molecules
    • Pharmacokinetic data analysis

Translational Research Laboratory Resources

  • Mesoscale (MSD) SECTOR® Imager 2400 multiplex analyzer
  • Maxwell 16 research system (automated DNA/RNA isolation)
  • Nanodrop 2.0 spectrophotometer
  • Leica phase contrast inverted microscope
  • Comet analysis electrophoresis system & Komet version 5.5 analysis Software
  • Refrigerated centrifuges & microcentrifuges
  • -70 C Freezers with integrated alarm systems
  • Laminar flow hoods and CO2 Tissue Culture Incubators

Pharmacology Laboratory Resources

  • Applied Biosystems API 3200 triple quadrupole tandem mass spectrometer
  • Shimadzu LC-20 series HPLC system
  • SPD-M20A photodiode array/RF-10AxL fluorescence detectors
  • SIL-20AC refrigerated autosampler
  • Agilent 6540 accurate-mass quadrupole time-of-flight (Q-TOF) mass spectrometer
  • Agilent 1290 ultra-high performance liquid chromatography (UPLC) system
  • Photodiode array (PDA) detector
  • Equipment for biological sample preparation and storage
  • Computers and data storage devices
  • Software for pharmacokinetic and pharmacodynamic analyses

Fees

Please click here to see fees. (PDF)


Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Translational Research Core Facility of the Case Comprehensive Cancer Center (P30 CA43703)."


Contact for Information

Seidman Cancer Center
Director: John J. Pink, PhD
216.368.5420
jrp16@case.edu
Operations Director: Paul Hartman, MS
216.844.1369
phh@case.edu
Manager: Erin Hohler
Seidman Cancer Center, Rm. 3608
216.286.3889/216.368.3890
fax 216.983.0815
emh14@case.edu
Cancer Pharmacology Lab
Co-Director (Pharmacology): Yan Xu, PhD
216.368.0315
Wolstein Research Building
yan.xu@case.edu
Taussig Cancer Institute
Director: Daniel J. Lindner, MD, PhD
216.445.0548
lindned@ccf.org
Manager: Marcella Mance
Taussig Cancer Institute R4-054
216.444.5393
mancem@ccf.org