Director: John J. Pink, PhD Assistant Professor, General Medical Sciences (Oncology) john.pink@case.edu Co-Director (Pharmacology): Yan Xu, PhD Professor, Chemistry yan.xu@case.edu CCF Site Director: Daniel J. Lindner, MD, PhD Assistant Professor, Cancer Biology lindned@ccf.org Operations Director: Paul Hartman, MS Manager of Dahms Clinical Research Unit Core Lab phh@case.edu

Services and Technologies

List of Services

1. Consultation (protocol, correlate, LOI, SOP, budget development)
2. Sample informatics and correlate data (databases)
3. Data analysis
4. Sample handling (receiving, storage, distribution)
5. Sample Processing (whole blood and paraffin block processing
   1. Dispensing whole blood aliquots
   2. Routine Blood Processing (PBMC, serum, or plasma isolation)
   3. DNA, RNA, protein extraction
   4. Flow or laser scanning cytometry staining
6. Lab Analysis
   1. Western Blots
   2. Standard ELISAs (Fluorescent or Luminescent platforms)
   3. MSD Multiplex Analysis (Mesoscale Electrochemiluminescence platform)
   4. PARP activity and Poly(ADP)-ribose assays
   5. Comet assays
7. Preclinical Drug Testing
   1. Single or multiple agent cytotoxicity (MTT or DNA content assays)
   2. Multiple agent analysis (Synergy analysis)
   3. Other single or multiple agent assays (e.g., flow cytometry)
8. Pharmacokinetic assay development
   1. Pharmacokinetic expertise for investigator-initiated early phase studies with planned PK analyses
   2. Sample procurement schedules for pharmacokinetic assays given anticipated or known PK profiles of investigational agents
   3. Design and development of analytical methods
   4. Adoption and validation of established analytical procedures
9. Pharmacokinetic assay performance
   1. Quantitative measurement of drugs, metabolites, and other small molecules
   2. Pharmacokinetic data analysis

Pharmacology Laboratory Resources

• Applied Biosystems API 3200 triple quadrupole tandem mass spectrometer coupled with a Shimadzu LC-20 series HPLC system along with SPD-M20A photodiode array/RF-10AxL fluorescence detectors and SIL-20AC refrigerated autosampler
• Agilent 6540 accurate-mass quadrupole time-of-flight (Q-TOF) mass spectrometer coupled with Agilent 1290 ultra-high performance liquid chromatography (UPLC) system and photodiode array (PDA) detector
• Equipment for biological sample preparation and storage
• Computers and data storage devices
• Software for pharmacokinetic and pharmacodynamic analyses

Fees

Please click here to see fees. (PDF)

Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Translational Research Core Facility of the Case Comprehensive Cancer Center (P30 CA43703)."

Contact for Information

Seidman Cancer Center

Director: John J. Pink, PhD 216.368.5420 john.pink@case.edu

Operations Director: Paul Hartman, MS 216.844.1369 phh@case.edu

Manager: Erin Hohler Seidman Cancer Center, Rm. 3608 216.286.3889/216.368.3890 fax 216.983.0815 emh14@case.edu

Cancer Pharmacology Lab

Co-Director (Pharmacology): Yan Xu, PhD 216.368.0315 Wolstein Research Building yan.xu@case.edu

Taussig Cancer Institute

Director: Daniel J. Lindner, MD, PhD 216.445.0548 lindned@ccf.org

Manager: Marcella Mance Taussig Cancer Institute R4-054 216.444.5393 mancem@ccf.org