TISSUE PROCUREMENT and HISTOLOGY CORE FACILITY (TPHC)
 |
 |
Director:
Gregory T. MacLennan, MD
Associate Professor, Pathology
gregory.maclennan@UHhospitals.org
Services
The Core facility supports Cancer Center member researchers by acquiring, preserving, and distributing high quality human tissues and providing priority histology services for both human and animal tissues.
Services available:
- Normal, benign, diseased, and malignant tissues from surgeries and autopsies
- Matched normal adjacent tissue and tissues from different organ sites of the same donor with special emphasis on accessing metastatic samples
- Tissues are prepared according to user-defined protocols and can be fresh, snap-frozen, or fixed
- Protocol development for tissue collection and processing
- Quality control reviews on human tissue samples are conducted to verify diagnosis before release to investigators (unless needed immediately)
- Demographic (age, race, gender) and histopathologic data supplied at no charge
- Long-term, monitored storage of samples (over 3 months)
- Consulting services on tissue availability and researcher application process
- Information source regarding Institutional Review Board (IRB) requirements for research uses of discarded human tissues and patient privacy regulations (HIPAA)
- Paraffin, OCT or methacrylate embedding
- Standard and serial sectioning
- Hematoxylin and eosin (H&E) and other morphological staining upon request
- Enzyme staining and immunohistochemistry (IHC)
- Laser capture microdissection (LCM)
Link to the Human Tissue Procurement Facility (HTPF) web page here
Please note: Researchers must complete a formal application and have IRB approval to access human tissue from the Core.
Resources
- Fully equipped laboratories for tissue collection, processing, and histology
- Three liquid nitrogen freezers including two K-Series Cryostorage Systems w/9,000 total sample capacity, equipped with Pacer 2L electronic monitoring and automated fill
- Leica 1850 Cryostat, several microtomes, LKB 2078 Histo Knife Maker, and related equipment for sectioning blocks and preparing slides
- Daily access to Tissue Tek VIP automatic tissue processor
- Olympus CH30 microscope equipped with D Plan Achromat objective lenses
- Arcturus PixCell IIe laser capture dissecting microscope
- Workstations for data collection and management; access to hospital databases
Fees
View the list of fees (PDF)
Contact for Information
| Locations: | Wearn Building, Rm. 333 (Tissue Procurement) |
| and Institute of Pathology, Rm. B-11 (Histology) | |
| Director: | Gregory T. MacLennan, MD |
| Phone: | 216/844-7452 |
| E-mail: | gtm2@case.edu |
| Director of Operations: | Robert E. Wyza, MS |
| Phone: | 216/844-5389 |
| Fax: | 216/844-4911 |
| E-mail: | rew7@case.edu |
| Histology Manager: | Nancy L. Edgehouse, BS |
| Phone: | 216/368-8700 |
| E-mail: | nle@case.edu |