Hematopoietic Biorepository and Cellular Therapy Core Facility

Scientific Director: David N. Wald, MD, PhD
Assistant Professor, Pathology
dnw@case.edu

  Stem Cell Group

Services

The Hematopoietic Biorepository and Cellular Therapy Core serves as a resource for procurement, processing, storage, banking, analysis, and distribution of cells derived from clinical-grade production, human blood, bone marrow, and umbilical cords.

The core is comprised of two components:

  1. A human hematopoietic biorepository and support services to facilitate basic hematopoietic research
  2. A clinical Cellular Therapy Lab that manufactures clinical-grade cell products for investigational and standard of care therapies

Facility Objectives

  • To procure, de-identify, process and distribute human blood, umbilical cord blood, and bone marrow cells from healthy volunteers and patients to investigators
  • To maintain a biorepository of collected samples
  • To perform and assist investigators with standard assays to evaluate hematopoiesis and hematopoietic and mesenchymal stem cells
  • To support cell therapy based clinical trials from the proof of concept stage throughout the clinical trial

List of Services

  1. Procurement and distribution of human hematopoietic cells:
    • The Core maintains an active IRB protocol to enable the recruitment of donors and procurement and distribution of de-identified blood and bone marrow samples to research investigators. We also maintain a Biorepository to supply investigators with cryopreserved samples. Sample types obtained include:
      • Normal donor blood
      • Normal donor bone marrow aspirate and/or biopsy samples
      • Umbilical cord blood
      • Peripheral mobilized hematopoietic progenitor cells
      • Bone marrow mesenchymal stem cells
      • Blood and/or bone marrow from patients with a wide variety of hematologic diseases including AML, ALL, CML, CLL, multiple myeloma, and anemia
  2. Processing and analysis of hematopoietic cells:
    • The Core assists investigators with the processing and analysis of hematopoietic cells using a variety of methodologies. Examples of some of these procedures include:
      • Separation of mononuclear cells by density gradient (Ficoll-Hypaque or Percoll)
      • Cell enrichment using CliniMACS magnetic bead separation (ex. CD34+ cell isolation)
      • Isolation and expansion of mesenchymal stem cells
      • Maintenance of specialized equipment available for research use including a hematology analyzer to perform CBCs
      • Performance of colony forming unit assays (CFU) on blood and bone marrow products
      • Separation of plasma/serum
  3. Production of clinical grade cellular therapy products
    • Isolation of specific cell subsets (CD34, CD3, CD14, etc )
    • Expansion of various cell types upon request
    • Ex-vivo lentiviral gene transfer
  4. Support capabilities to transition preclinical studies into clinical trials
    • Assistance in the design and preparation of cells for Good Laboratory Practices (GLP) safety studies
    • Investigational New Drug Application (IND) preparation
    • Sourcing of clinical grade supplies and reagents
    • Scale up from research to clinical operations, including preparation of investigational clinical therapy grade cellular products
    • GMP training to lab investigators

Resources

Hemavet Hematology Analyzer
Guava Cytometer
Biorad QPCR machine
ISO 7 cleanroom suites (6) each with incubators, centrifuge, biosafety cabinet, microscope, refrigerator
GMP-trained lab personnel
COBE closed system cell washer
Rate controlled freezing chamber
Clinical grade Cytokine stocks: Interleukins 3 and 6, stem cell factor and Flt-3 ligand, thrombopoietin
VarioMACS columns/antibody cocktails for routine separation of CD34+ cells and other cell subsets
Shandon Cytospin


Fees

List of Fees Under Construction


Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Hematopoietic Biorepository and Cellular Therapy Core Facility of the Case Comprehensive Cancer Center (P30CA043703)."


Contact for Information

Location: Wolstein Research Building, 6-303

Core Contact: Basabi Maitra
216.368.1495
basabi.maitra@case.edu

Scientific Director: David N. Wald, MD, PhD
216.368.5668
dnw@case.edu

Operations Director: Jane Reese Koç, MBA, MT
216.368.1007
jsr5@case.edu