Proteomics Core Facility

Dr. Chance Proteomics Core

Director: Mark R. Chance, PhD
Professor, General Medical Sciences
mark.chance@case.edu

Assistant Director: Janna Kiselar, PhD
Instructor, Center for Proteomics and Bioinformatics
janna.kiselar@case.edu

Co-Director: Belinda B. Willard, PhD
Staff, Cell Biology
willarb@ccf.org


Services

The overall goal of the Proteomics Core Facility is to make advanced mass spectrometry instruments, expertise, and methods for proteome analysis available to all investigators.

Services include the following:

  • Protein Identification
  • Characterization of post-translational modifications
  • Quantitation of protein expression
    • Quantitative Top-Down Proteomics using 2-D gels
    • Quantitative Bottom-Up Proteomics using LC-MS
    • Experimental Design for Quantitative Proteomics
    • Data analysis
  • Bioinformatics analysis
  • Training
  • Independent use
  • Interactome analysis
  • Structural analysis (protein footprinting)

Resources

Case Western Reserve University facility:

  • Two Thermo Scientific Orbitrap Elite with a Waters nanoACQUITY UPLC system
  • Thermo Scientific Fourier Transform LTQ mass spectrometer with high resolution and ppm mass accuracy for top-down and bottom-up proteomics capabilities operated with a Dionex UltiMate 3000 Rapid Separation LC (RSLC)
  • Thermo Scientific LTQ Orbitrap XL ETD with a Waters nanoACQUITY UPLC system
  • Thermo Scientific LTQ Orbitrap Velos with a Waters nanoACQUITY UPLC system
  • Thermo Scientific TSQ Quantum Ultra Triple Quad Mass Spectrometer with a Dionex UltiMate 3000 Rapid Separation LC (RSLC)
  • prOTOF 2000 MALDI

Lerner Research Institute facility:

  • ThermoFinnigan LTQ
  • ThermoFinnigan LTQ-Orbitrap Elite

Further Information

Case Western Reserve University
Center for Proteomics and Bioinformatics
http://proteomics.case.edu/

Cleveland Clinic Lerner Research Institute
Proteomics Laboratory
http://www.lerner.ccf.org/services/mass_spec/


Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Proteomics Core Facility of the Case Comprehensive Cancer Center (P30CA043703)."


Contact for Information

CWRU FACILITY   LRI FACILITY
Location: Case BRB 9th floor   Location: LRI Room NN1-25
Director: Mark R. Chance, PhD
216.368.4406
mark.chance@case.edu
  Co-Director: Belinda B. Willard, PhD
216.444.7170
willarb@ccf.org
General Contact: Janna Kiselar, PhD
Assistant Director
216.368.1490
proteomics@case.edu