CYTOMETRY CORE FACILITY
Director: James W. Jacobberger, PhD
Professor, General Medical Sciences (Oncology)
jwj@case.edu
Services
The Cytometry core provides flow and image cytometry and cell sorting instrumentation, expertise, training, consultation, and services to Cancer Center investigators. Services provided:
- Consultation
- Training
- Teaching
- Flow Cytometry - low, intermediate and high levels
- Image Cytometry
- Cell Sorting
- Cell Counting, live/dead measurements
- Data Analysis
- Staining
Instrumentation
Cell Sorters:
- BD Biosiences FACSAria: 3 laser, 9 parameter, high speed, 4 way, auto-cloning, ~biosafety II
- Beckman Coulter Elite ESP: 2/3 laser, 8 parameter, normal speed, 2 way, auto-cloning
Cell Analyzers:
- BD Biosiences LSR: 3 laser, 8 parameters (6 color)
- BD Biosiences LSR 2: 4 laser, 42 parameters (12 color)
- Beckman Coulter XL: 1 laser, 8 parameter (4 color)
Compucyte iCyte:
- 3 laser, solid support scanning cytometer, (6 parameter, 5 color)
Resources and Fees
Fees are charged for data acquisition time only. Data analysis, graphics, etc. are charged at the same rate, if Core personnel perform the function. Routine consultation is not charged.
Fees*
| Cell Sorting | |
|---|---|
| BD FACSAria | 110.00/Hour |
| BC Elite ESP | 100.00/Hour |
| Analyzers | |
| BD LSR I | 75.00/Hour |
| BD LSR II | 95.00/Hour |
| BC XL-MCL | 60.00/Hour |
| Counters | |
| Guava EasyCyte** | 1.00/Test |
| Laser Scanning Cytometer | |
| Compucyte iCyte | By arrangement with Core Director or Staff |
*Non Cancer Center Members are charged an additional 30% / Hour.
**Guava EasyCyte reagents provided at additional cost.
Contact for Information
| Location: | Wolstein Research Building-3rd Floor, Cell Sorters-3515, Cell Analyzers-3517 also at the Lerner Research Institute |
|---|---|
| Director: | James W. Jacobberger, PhD |
| Phone: | 216/368-4645 |
| E-mail: | jwj@case.edu |
| Manager: | R. Michael Sramkoski |
| Phone: | 216/368-1021 |
| E-mail: | rms19@case.edu |