Director: Martina L. Veigl, PhD Associate Professor, General Medical Sciences (Oncology) mlv2@case.edu

Link to the Gene Expression & Genotyping Core Facility home page: http://www.gegf.net.

Services

The Gene Expression & Genotyping Facility (GEGF) facilitates implementation of high throughput genetic technologies for the research conducted by members of the Case community.

Primary Services

Gene Expression (Affymetrix)

• Brokerage of microarrays (testchips and full expression arrays)
• cRNA preparation
• Evaluation of cRNA quality on test arrays
• Processing user-prepared and/or facility-prepared cRNA microarrays
• Data reduction/data analysis
• Low cost access to valuable, advanced, server-based data mining and annotation tools

Gene Expression (RT PCR)

• Brokerage of ABI RT PCR assay kits and reagents
• Sample preparation
• RT PCR set-up for relative quantitation and absolute quantitation
• Execution of RT PCR for user-prepared and/or facility-prepared PCR reactions
• Help with data interpretation/analysis
• Endogenous control determinations

SNP Analysis (Affymetrix)

• Brokerage of 10K and 100K SNP arrays
• Sample preparation
• Processing of sample on Affymetrix SNP array
• Help with analysis

SNP Analysis (ABI)

• Sample prep (384 well format, high throughput enabled by Matrix robotics)
• Endpoint plate reading (high throughput enabled by ABI autoloader)
• Measurement of real time spectral data (initial validations) on 7900
• Basic PCR execution on 9700
• Matrix robotics (programmable for custom set-up)
• Help with analysis

Accurate RNA quality assessment and quantitation

• Accurate quantitation of RNA samples on Nanodrop spectrophotometer
• Quality assessment on Agilent Bioanalyzer

Additional Services

• Individually tailored consultation with potential users
• Assistance with grant preparation
• Assistance with experimental design
• Facilitate monthly meetings of Working User's Group to discuss experimental design, data reduction, etc.
• Serve as resource for linking users to databases, expression array data, etc.
• Provide 3 credit hour course on issues pertaining to expression arrays
• Regular software workshops/training

Fees

Visit http://www.gegf.net to obtain fees for Arrays and Services.

Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Gene Expression & Genotyping Core Facility of the Case Comprehensive Cancer Center (P30 CA43703)."

Contact for Information

Location:	Wolstein Research Building, 3-401
Director:	Martina L. Veigl, PhD
Phone:	216.368.5689
E-mail:	mlv2@case.edu
Manager:	Debora Poruban
Phone:	216.368.8748
E-mail:	debora.poruban@case.edu