GENE EXPRESSION and GENOTYPING CORE FACILITY
Director:
Martina L. Veigl, PhD
Associate Professor, General Medical Sciences (Oncology)
mlv2@case.edu
Link to the Gene Expression and Genotyping Core Facility home page: http://www.gegf.net.
Services
The Gene Expression and Genotyping Facility (GEGF) facilitates implementation of high throughput genetic technologies for the research conducted by members of the Case community.
Primary Services
Gene Expression (Affymetrix)
- Brokerage of microarrays (testchips and full expression arrays)
- cRNA preparation
- Evaluation of cRNA quality on test arrays
- Processing user-prepared and/or facility-prepared cRNA microarrays
- Data reduction/data analysis
- Low cost access to valuable, advanced, server-based data mining and annotation tools
- Brokerage of ABI RT PCR assay kits and reagents
- Sample preparation
- RT PCR set-up for relative quantitation and absolute quantitation
- Execution of RT PCR for user-prepared and/or facility-prepared PCR reactions
- Help with data interpretation/analysis
- Endogenous control determinations
- Brokerage of 10K and 100K SNP arrays
- Sample preparation
- Processing of sample on Affymetrix SNP array
- Help with analysis
- Sample prep (384 well format, high throughput enabled by Matrix robotics)
- Endpoint plate reading (high throughput enabled by ABI autoloader)
- Measurement of real time spectral data (initial validations) on 7900
- Basic PCR execution on 9700
- Matrix robotics (programmable for custom set-up)
- Help with analysis
- Accurate quantitation of RNA samples on Nanodrop spectrophotometer
- Quality assessment on Agilent Bioanalyzer
Additional Services
- Individually tailored consultation with potential users
- Assistance with grant preparation
- Assistance with experimental design
- Facilitate monthly meetings of Working User's Group to discuss experimental design, data reduction, etc.
- Serve as resource for linking users to databases, expression array data, etc.
- Provide 3 credit hour course on issues pertaining to expression arrays
- Regular software workshops/training
Fees
Visit http://www.gegf.net to obtain fees for Arrays and Services. There is a 30% discount for Cancer Center members.
Contact for Information
| Location: | Wolstein Research Building, 3-401 |
|---|---|
| Director: | Martina L. Veigl, PhD |
| Phone: | 216/368-5689 |
| E-mail: | mlv2@case.edu |
| Manager: | Debora Poruban |
| Phone: | 216/368-8748 |
| E-mail: | debora.poruban@case.edu |