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Services

Tissue Procurement and Annotation

  • Tissue Procurement team works directly with surgical pathologists to obtain samples
  • Custom protocol development for tissue collection and processing
  • Quality control reviews on human tissue samples are conducted to verify diagnosis before release to investigators (unless needed immediately)

Types of Tissue Available

  • Normal, benign, diseased, and malignant tissues from surgeries and autopsies
  • Matched normal adjacent tissue and tissues from different organ sites of the same donor with special emphasis on accessing metastatic samples
  • Tissues are collected from over 50 anatomic sites
  • No samples are collected from individuals with known infectious illnesses
  • Fetal biospecimens are not collected due to state and local statutes

Standard Data

  • Patient confidentiality is strictly maintained through the use of sample code numbers
  • Coded Surgical Pathology or Autopsy Reports containing final diagnosis
  • Tissue type and histopathologic status, sample weight or dimensions
  • Demographics (age in years, race, gender)

Additional Data Available

  • Chart reviews performed upon request to obtain additional clinical information
  • De-linked specimens are not traceable, and as such no further information can be obtained for these samples.

Tissue Processing

  • Preparations according to user-defined protocols
  • Frozen specimens, OCT-embedded and paraffin-embedded tissues, sections, cores, and stained or unstained slides
  • Freezing in liquid or vapor phase of liquid nitrogen
  • Sterile dissection
  • RNA preservation
  • Custom QC sampling

Biospecimen Banking

  • Collection, annotation and storage of remnant human tissues, blood and other body fluids targeted towards cancer and other medical research, for current and future research projects
  • Long-term, controlled storage of biospecimens for specific researchers
  • Access to QC research blocks maintained by the HTPF
  • Peripheral blood and bone marrow samples from initial visits and follow-up procedures processed by Hematopoietic Stem Cell Core to obtain serum and cell fractions for storage

View availTMA in the biorepository

Access to Archived Clinical Blocks

  • Access to UHCMC clinical archives of paraffin blocks

Tissue Microarrays (TMAs)

  • Custom design and production of Tissue Microarrays

View biorep_samples in the biorepository

Histology

  • Fixation of researcher-supplied tissues
  • Paraffin, OCT or methacrylate embedding
  • Serial sectioning and custom orientations
  • Unstained slides, Hematoxylin and Eosin (H&E) staining, special morphological stains, and enzyme histochemistry available

Immunohistochemistry (IHC), Immunofluorescence and In situ hybridization

  • Routine immunohistochemistry and protocol development of commercially available and novel antibodies
  • Over 100 IHC assays available
  • In situ hybridization service

Microscopy and Digital Imaging

  • Light, fluorescence, and phase contrast microscopy
  • Digital imaging for Histology, IHC, & TMA prepared slides

Laser Capture Microdissection (LCM)

  • State-of-the-art LCM
  • Extremely versatile fluorescence capabilities
  • Achieve near 100% purity in the dissection and procurement of targeted tissue

Consultation and Training

  • Consulting services on tissue availability for different disease areas and what can be expected in terms of sample size, availability of matched normal tissues, and matched primary and metastatic tissues form the same donor
  • Information source regarding Institutional Review Board (IRB) requirements for research uses of discarded human tissues and patient privacy regulations (HIPAA)
  • Assistance throughout application process for access to biospecimens
  • Advice on histology and IHC stains that may be appropriate for a particular study
  • TMA design including cancer subtypes, positive and negative controls, physical layouts
  • Training on use of microtomes and cryostats, which are available on a rental basis
  • Consultation and training on laser capture microdissection

Director

Marta Couce, MD
Professor, Pathology
marta.couce@UHhospitals.org

Associate Director of IHC

Dawn M. Dawson, MD
Assistant Professor, Pathology
dmd2@case.edu

Medical Advisor

Joseph E. Willis, MD
Associate Professor, Pathology
joseph.willis@case.edu