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Hematopoietic Biorepository & Cellular Therapy

Services

The Hematopoietic Biorepository and Cellular Therapy Core serves as a resource for procurement, processing, storage, banking, analysis, and distribution of cells derived from clinical-grade production, human blood, bone marrow, and umbilical cords.

The core is comprised of two components:

  1. A human hematopoietic biorepository and support services to facilitate basic hematopoietic research
  2. A clinical Cellular Therapy Lab that manufactures clinical-grade cell products for investigational and standard of care therapies

Facility Objectives

  • To procure, de-identify, process and distribute human blood, umbilical cord blood, and bone marrow cells from healthy volunteers and patients to investigators
  • To maintain a biorepository of collected samples
  • To perform and assist investigators with standard assays to evaluate hematopoiesis and hematopoietic and mesenchymal stem cells
  • To support cell therapy based clinical trials from the proof of concept stage throughout the clinical trial

List of Services

  1. Procurement and distribution of human hematopoietic cells:
    • The Core maintains an active IRB protocol to enable the recruitment of donors and procurement and distribution of de-identified blood and bone marrow samples to research investigators. We also maintain a Biorepository to supply investigators with cryopreserved samples. Sample types obtained include:
      • Normal donor blood
      • Normal donor bone marrow aspirate and/or biopsy samples
      • Umbilical cord blood
      • Peripheral mobilized hematopoietic progenitor cells
      • Bone marrow mesenchymal stem cells
      • Blood and/or bone marrow from patients with a wide variety of hematologic diseases including AML, ALL, CML, CLL, multiple myeloma, and anemia
  2. Processing and analysis of hematopoietic cells:
    • The Core assists investigators with the processing and analysis of hematopoietic cells using a variety of methodologies. Examples of some of these procedures include:
      • Separation of mononuclear cells by density gradient (Ficoll-Hypaque or Percoll)
      • Cell enrichment using CliniMACS magnetic bead separation (ex. CD34+ cell isolation)
      • Isolation and expansion of mesenchymal stem cells
      • Maintenance of specialized equipment available for research use including a hematology analyzer to perform CBCs
      • Performance of colony forming unit assays (CFU) on blood and bone marrow products
      • Separation of plasma/serum
  3. Production of clinical grade cellular therapy products
    • Isolation of specific cell subsets (CD34, CD3, CD14, etc )
    • Expansion of various cell types upon request
    • Ex-vivo lentiviral gene transfer
  4. Support capabilities to transition preclinical studies into clinical trials
    • Assistance in the design and preparation of cells for Good Laboratory Practices (GLP) safety studies
    • Investigational New Drug Application (IND) preparation
    • Sourcing of clinical grade supplies and reagents
    • Scale up from research to clinical operations, including preparation of investigational clinical therapy grade cellular products
    • GMP training to lab investigators

Resources

Hemavet Hematology Analyzer
Guava Cytometer
Biorad QPCR machine
ISO 7 cleanroom suites (6) each with incubators, centrifuge, biosafety cabinet, microscope, refrigerator
GMP-trained lab personnel
COBE closed system cell washer
Rate controlled freezing chamber
Clinical grade Cytokine stocks: Interleukins 3 and 6, stem cell factor and Flt-3 ligand, thrombopoietin
VarioMACS columns/antibody cocktails for routine separation of CD34+ cells and other cell subsets
Shandon Cytospin

Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications: 
"This research was supported by the Hematopoietic Biorepository and Cellular Therapy Core Facility of the Case Comprehensive Cancer Center (P30CA043703)."

Contact for Information

Location: Wolstein Research Building, 6-303

Core Contact: Basabi Maitra
216.368.1495
basabi.maitra@case.edu

Scientific Director: David N. Wald, MD, PhD
216.368.5668
dnw@case.edu

Operations Director: Jane Reese Koç, MBA, MT
216.368.1007
jsr5@case.edu

Director

David N. Wald, MD, PhD
Assistant Professor, Pathology
dnw@case.edu