CWRU Links
Athymic Animal & Xenograft

Services

The Athymic Animal & Xenograft Core consists of two physical sites, one at Case Western Reserve University and one at Cleveland Clinic at the Taussig Cancer Institute. The purpose of the core is to help principal investigators of CWRU and Cleveland Clinic perform tumor experiments, angiogenesis studies, and pharmacokinetic studies in mice. The Core routinely conducts animal experiments involving several syngeneic murine tumor models, and several human xenograft (athymic nude mouse, and NSG – NOD scid IL2Rg mouse) tumor models. In addition, the Core has developed several orthotopic tumor models: brain, lung, breast, renal, spleen, skin, ovarian, prostate, bladder, and pancreas. The Core is always willing to develop additional new models.

Core services utilize murine models to facilitate in vivo cancer drug screening, drug evaluation and drug development. Core services include drug administration (sub-cutaneous, intravenous, intraperitoneal, prolonged slow-release silastic capsule), blood drawing (repetitive - saphenous vein, or terminal – cardiac puncture) for pharmacokinetic pharmacodynamic (PK/PD) studies, absorption distribution metabolism excretion (ADME) studies, syngeneic and xenograft tumor models, orthotopic tumor models. The goal is to help in evaluation of new pharmacologic agents by providing data on tumor growth, metastasis, angiogenesis, and gene expression. The facility also provides consultation services to assist the investigator in all aspects of animal tumor model development.

Resources

  • Laminar flow work station
  • Laboratory work room containing two laminar flow hoods and a type B, 100% exhaust biohazard hood to accommodate preparation of cancer cells, anticancer agents, and other pharmaceutical agents
  • Full complement of stainless steel surgical instruments
  • Pass-through autoclave connecting the three animal rooms
  • Steris "super-autoclave" installed adjacent to the facility as a backup

Fees

NSG mouse (NOD/SCID/IL2Rgamma) breeding:

  • $85 per mouse

Athymic mouse (NCR nu/nu) breeding:

  • $40 per mouse (*new rate as of Dec 1, 2014)

Athymic mouse (NOD/SCID) breeding:

  • $65 per mouse  (*new rate as of Dec 1, 2014)

Animal housing, mice:

  • $1.10/cage/day

Animal housing, rats:

  • $1.30/cage/day

Experimental techniques training:

  • $25.00/person/hour

Services Provided at CWRU

  • All services performed at $30/hour
  • Drug administration including intravenously (IV), intramuscularly (IM), intraperitonealy (IP), and subcutaneously (SC)
  • Blood draw including cardiac and retro-orbital
  • Tumor cell injection including cardiac and tail vein
  • Xenografts: including subcutaneous or orthotopic transplantation into a variety of organs. These include mammary fat pad, ovary, oviduct, kidney, pancreas, and brain
  • Mouse weight and tumor caliper measurements
  • Delivery and imaging of luciferase-labeled tumors
  • Organ and tissue harvest
  • Assistance in preparing IACUC protocols for individual laboratories

Services Provided at the Taussig Cancer Institute, Cleveland Clinic

  • Blood Draw
    • Cardiac puncture: $8.55
    • Retro-orbital: $6.09
  • Dermal Assay: $16.54
  • Digital Photograph Image Service: $5.83
  • Drug Administration
    • IM: $1.26
    • IP: $1.26
    • IV: $3.68
    • SQ: $1.26
    • PO: $1.26
    • Sterile: $4.88
  • Measure of Tumor (MWF for duration of study): $12.13
  • Measure of Mouse Weight (every Wed for duration of study): $6.09
  • Miscellaneous: $1.10
  • Organ & Tissue Harvest
    • Formalin or OCT: $6.09
    • Liquid Nitrogen: $8.61
  • Tech time (per hour): $25.00
  • Total Body Radiation: $1.26
  • Tumor cell inoculations
    • Orthotopic: $8.55
    • Subcutaneous Xenograft or Syngraft: $2.00
  • Tumor cell line expansions: $30.40
  • Xenogen Imaging: $11.60
  • Credit Record: $1.00

Acknowledgement

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Athymic Animal and Xenograft Core Facility of the Case Comprehensive Cancer Center (P30CA043703)."

Contact for Further Information

CWRU FACILITYTAUSSIG FACILITY
Location: School of Medicine, East B21, 22, 23
Wolstein Research Bldg, SB162
Location: Lerner Research Institute, NC4-12B
Manager: Kristen Lozada
216.368.5924
klw9@case.edu
Director: Daniel J. Lindner, MD, PhD
216.445.0548
lindned@ccf.org
Experimental Design Consultant: Ruth Keri, PhD
216.368.5924
keri@case.edu
Research Technologist: Yvonne Parker
parkery2@ccf.org
Veterinary Services: W. John Durfee, DVM
216.368.3940
jwd7@case.edu
 
Core Technician: Pamela Steele
216.368.3169
pas@case.edu
 

Director

Daniel J. Lindner, MD, PhD
Assistant Professor, Cancer Biology
lindned@ccf.org