GENERAL MEDICAL SCIENCES (ONCOLOGY)
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JAMES W. JACOBBERGER, PhDProfessor Associate Director for Shared Resources Fax: 216/368-8919 email: jwj@case.edu |
Research Interests
I am generally interested in mammalian cell biology and cancer cell biology. I was raised in Departments of Microbiology (B.S., University of Nebraska, 1976; Ph.D., University of Rochester, 1983) and Pathology (Post Doc, University of Colorado Health Sciences Center 1983-85) and therefore have those cultural orientations. I have specialized in Cytometry as a scientific discipline (i.e., not as a tool) from graduate school onwards. Current focus areas are cell signaling and cell cycle processes viewed from a systems orientation.
We have two, funded, main enterprises in the lab: multi-variate cell cycle analysis and modeling, and leukemia cell signaling. There is a single overarching goal – to test the following set of ideas. Cytometric measurements of biochemistry resolve to kinetic activity profiles. Well-chosen multi-variate measurements resolve precisely and completely. Gated bivariate views or an N-dimensional view of these profiles produce rule-based, quantifiable/classifiable discrete or finite cell states – some may be trivial, many are not. In many cases, these states equate to cell behavior/function. Cytometrists know the bivariate data as negative and positive clusters/populations. Mathematic modelers of biochemistry know the profiles as inputs and outputs. The long range goal here (as yet unfunded) is to create an analytical system, preferably within a clinical pathology or observational basic cell biology modes of investigation, in which the measurements made at the cytometer are interactively interrogated by the investigator and a computer program with mathematical models of cell biochemistry and integrated systems simulations running underneath.
Selected Publications
Jacobberger JW. Increasing the power of cytometry. Nat Methods 3:343-344, 2006.
Darzynkiewicz Z, Crissman H, Jacobberger JW. Cytometry of the cell cycle: cycling through history. Cytometry A 58:21-32, 2004.
Jacobberger JW, Frisa PS, Sramkoski RM, Stefan T, Shults KE, Soni DV. A new biomarker for mitotic cells. Cytometry A 73A:5-15, 2008.
Hastak K, Paul RK, Agarwal MK, Thakur VS, Amin AR, Agrawal S, Sramkoski RM, Jacobberger JW, Jackson MW, Stark GR, Agarwal ML. DNA synthesis from unbalanced nucleotide pools causes limited DNA damage that triggers ATR-CHK1-dependent p53 activation. Proc Natl Acad Sci USA 105:6314-6319, 2008.
Soni DV, Sramkoski RM, Lam M, Stefan T, Jacobberger JW. Cyclin B1 is rate limiting but not essential for mitotic entry and progression in mammalian somatic cells. Cell Cycle 7:1285-1300, 2008.
Soni DV, Jacobberger JW. Inhibition of Cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from Mid G(2) through prophase. Cell Cycle 3:349-357, 2004.
Yan T, Desai AB, Jacobberger JW, Sramkoski RM, Loh T, Kinsella TJ. CHK1 and CHK2 are differentially involved in mismatch repair-mediated 6-thioguanine-induced cell cycle checkpoint responses. Mol Cancer Ther 3:1147-1157, 2004.
Crosby ME, Jacobberger J, Gupta D, Macklis RM, Almasan A. E2F4 regulates a stable G(2) arrest response to genotoxic stress in prostate carcinoma. Oncogene 26:1897-1909, 2007.
Jacobberger JW, Sramkoski RM, Frisa PS, Ye PP, Gottlieb MA, Hedley DW, Shankey TV, Smith BL, Paniagua M, Goolsby CL. Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity. Cytometry A 54:75-88, 2003.
Briggs RC, Shults KE, Flye LA, McClintock-Treep SA, Jagasia MH, Goodman SA, Boulos FI, Jacobberger JW, Stelzer GT, Head DR. Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes: evidence for a role in apoptosis. Cancer Res 66:4645-4651, 2006.
Chow S, Hedley D, Grom P, Magari R, Jacobberger JW, Shankey TV. Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A 67:4-17, 2005.