CWRU Links
Cytometry Instrumentation

Cell Sorting

  • BD Biosciences Aria
    • 3 lasers; 9 color (blue, green, orange, red, far-red) emission
    • Compatible dyes: Hoechst 333 42DAPIPacific BluePacific Orange
      Fluorescein/A488GFPPIAO7AADPEA555Cy3YFPPE-Cy5PE-Cy5.5, 
      PE-Cy7
      PerCpPerCp-Cy7A647A700APAP-Cy7
    • Four way sorting; multi-well plate sorting. Sort speed 25,000/s
  • ICyte Reflection
    • 5 lasers (choice of 2); 4 color (blue, green, orange, red, far-red) emission
    • Compatible dyes: Hoechst 33342DAPIPacific BluePacific OrangeFluorescein/A488
      GFPPIAO7AADPEA555Cy3YFPPE-Cy5PE-Cy5.5PE-Cy7PerCpPerCp-Cy7
      Texas RedRFPA647A700APAP-Cy7
    • Four way sorting. Flexible, high speed. Sort speed 50,000/s

Flow Cytometry

  • Millipore Guava
    • 1 blue laser; 2 color (green and red) emission
    • Compatible dyes: Fluorescein/A488PI7AAD
    • This cytometer is mainly used for live/dead cell counting and data are output as events per ml.
      Does not have light scatter parameters.
  • Beckman Coulter XL
    • 1 blue laser; 4 color (green, orange, red, far red) emission
    • Compatible dyes: Fluorescein/A488GFPPIAO7AADPEA555Cy3YFPPE-Cy5PE-Cy5.5
      PE-Cy7PerCpPerCp-Cy7
  • BD Biosciences LSR 2
    • 5 lasers; 14 color (blue, green, orange, red, far red) emission
    • Compatible dyes: Hoechst 33342DAPIA350Pacific BluePacific OrangeFluorescein/A488GFP
      PIAO7AADPEA555Cy3YFPPE-Cy5PE-Cy5.5PE-Cy7PerCpPerCp-Cy7A647A700APAP-Cy7

Imaging Cytometry

  • Compucyte iCyte laser scanning cytometer (LSC)
    • 3 lasers; 8 color (blue, green, orange, red) emission
    • Compatible dyes: Hoechst 33342DAPIPacific BluePacific OrangeFluorescein/A488GFP
      PIAO7AADPEA555Cy3YFPPE-Cy5PerCpA647AP
    • This platform accepts chamber slides, standard culture dishes with coverslipped
      windows (Labtek); optical grade multiwall plates
  • Amnis Image Stream flow cytometer
    • 3 lasers; X color (blue, green, orange, red) emission
    • Compatible dyes: Hoechst 33342DAPIPacific BluePacific OrangeFluorescein/A488
      GFPPIAO7AADPEA555Cy3YFPPE-Cy5PE-Cy5.5PE-Cy7PerCpPerCp-Cy7A647AP

Director

James W. Jacobberger, PhD
Professor, General Medical Sciences (Oncology)
jwj@case.edu

Online Scheduling Available for Experienced Users

Experienced users may schedule time on the appropriate instrument using the Ca-MiMi online scheduler.